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kapa library quantification kit  (Roche)


Bioz Manufacturer Symbol Roche manufactures this product  
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    Structured Review

    Roche kapa library quantification kit
    Kapa Library Quantification Kit, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kapa+library+quantification+kit/KAPA+Quantification+Kit/pmc12854873-165-8-12
    Average 99 stars, based on 5 article reviews
    kapa library quantification kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Library Quantification:

    Article Title: Effect of Zearalenone on Microbial Spatial Differences in the Chyme and Intestinal Mucosa of Piglets.
    Article Snippet: .. Library size distribution was assessed on an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), and quantification was performed using the Kapa Library Quantification Kit (Kapa Biosystems, Wilmington, MA, USA). .. Paired-end sequencing (2250 bp) was conducted on an Illumina NovaSeq 6000 platform (LC-Bio, Hangzhou, Zhejiang, China).

    Article Title: Exploring CSF microRNA signatures as diagnostic biomarkers in adult-type diffuse gliomas
    Article Snippet: .. Pooled libraries were quantified by qPCR using the KAPA Library Quantification Kit (KAPA Biosystems). .. The Illumina NextSeq 500 was used to sequence the pooled library, one pool per run, in a single-end configuration, with a read length of 75 bp.

    Article Title: Whole-exome sequencing unveils novel potential gene mutations involved in primary renal small cell carcinoma
    Article Snippet: .. The concentration was accurately measured using the Qubit ® dsDNA HS Assay Kit (Thermo Fisher Scientific) and confirmed by quantitative PCR (qPCR) using the KAPA Library Quantification Kit (Roche, Catalog No. KK4824) to ensure precise molarity calculation for cluster generation. ..

    Article Title: Cell culture methods and compositions
    Article Snippet: To remove excess primers and adaptor dimers, PCR products were purified twice using SPRI beads (Beckman Coulter). .. The quality of the libraries was assessed using Agilent Bioanalyzer DNA 1000 chip (Duke-NUS Genome Facility) and the molar concentration of each library was determined using KAPA library quantification kit (Roche). .. Three pooled libraries (each containing 9 samples with the pooled concentration of 8 nM in 30 uL) was sent for sequencing on Illumina HiSeq4000 (NovogeneAIT Genomics Singapore).

    Article Title: Single-cell eQTL mapping reveals convergent glial–neuronal risk architecture in Parkinson’s disease
    Article Snippet: Indexed sequencing libraries were constructed using the Single-Cell 3′ Library Construction Kit v3.1 (10x Genomics, #PN-1000190) and Dual index kit TT set A (10x Genomics, #PN-1000215), as per the manufacturer’s protocol. .. The barcoded single nuclei libraries were quantified by quantitative PCR using KAPA Library Quantification Kit (KAPA Biosystems/Roche, #7960336001 and kit code-KK4873). .. Next, the libraries were sequenced using NextSeq 500/550 High Output Kit v2.5 150 Cycles (Illumina, #20024907) on an Illumina NextSeq 550 platform with following run parameters: Read 1: 28; i7 index: 10; i5 index: 10; Read2: 120.

    Article Title: A Very-low-energy Fast Involves Increased Adipose Inflammatory Gene Expression: A 6-day Feeding Trial (FASTOMICS-6)
    Article Snippet: .. The final cDNA libraries were additionally quantified with the KAPA Library Quantification Kit (Roche, USA) for Illumina sequencing platforms. .. All samples were paired-end sequenced (2 × 100 bp) on the Illumina NovaSeq 6000 system (Illumina, San Diego, CA, USA).

    Article Title: Hypoxic CAF studies unveil PTHrP-vitamin D-RAS axis as pivotal in the CAF
    Article Snippet: Sequencing was performed using the NovaSeq 6000 S1 Reagent Kit v1.5 (200 cycles; cat. no. 20028318; Illumina, Inc.). .. Final library concentrations were quantified using the KAPA Library Quantification Kit (cat. no. KK4824/D; Roche Diagnostics) by real-time PCR. ..

    Article Title: A combinatorial domain screening platform reveals epigenetic effector interactions for transcriptional perturbation.
    Article Snippet: For one 50 μL of the PCR reaction, 25 μL of 2X NEBNext HiFi (NEB), 1 μg of gDNA, and a final concentration of 0.5 μM for each forward and reverse NGS primers AR TI CL E IN P RE SS (Supplementary Data 9) were prepared and mixed on ice. .. The gel-extracted library was quantified by Nanodrop (Thermo Fisher Scientific), Qubit HS kit (Thermo Fisher Scientific), Bioanalyzer (Agilent Technologies), and KAPA Library Quantification Kit (Roche). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Exploring CSF microRNA signatures as diagnostic biomarkers in adult-type diffuse gliomas
    Article Snippet: .. Pooled libraries were quantified by qPCR using the KAPA Library Quantification Kit (KAPA Biosystems). .. The Illumina NextSeq 500 was used to sequence the pooled library, one pool per run, in a single-end configuration, with a read length of 75 bp.

    Article Title: Whole-exome sequencing unveils novel potential gene mutations involved in primary renal small cell carcinoma
    Article Snippet: .. The concentration was accurately measured using the Qubit ® dsDNA HS Assay Kit (Thermo Fisher Scientific) and confirmed by quantitative PCR (qPCR) using the KAPA Library Quantification Kit (Roche, Catalog No. KK4824) to ensure precise molarity calculation for cluster generation. ..

    Article Title: Single-cell eQTL mapping reveals convergent glial–neuronal risk architecture in Parkinson’s disease
    Article Snippet: Indexed sequencing libraries were constructed using the Single-Cell 3′ Library Construction Kit v3.1 (10x Genomics, #PN-1000190) and Dual index kit TT set A (10x Genomics, #PN-1000215), as per the manufacturer’s protocol. .. The barcoded single nuclei libraries were quantified by quantitative PCR using KAPA Library Quantification Kit (KAPA Biosystems/Roche, #7960336001 and kit code-KK4873). .. Next, the libraries were sequenced using NextSeq 500/550 High Output Kit v2.5 150 Cycles (Illumina, #20024907) on an Illumina NextSeq 550 platform with following run parameters: Read 1: 28; i7 index: 10; i5 index: 10; Read2: 120.

    Article Title: Hypoxic CAF studies unveil PTHrP-vitamin D-RAS axis as pivotal in the CAF
    Article Snippet: Sequencing was performed using the NovaSeq 6000 S1 Reagent Kit v1.5 (200 cycles; cat. no. 20028318; Illumina, Inc.). .. Final library concentrations were quantified using the KAPA Library Quantification Kit (cat. no. KK4824/D; Roche Diagnostics) by real-time PCR. ..

    Concentration Assay:

    Article Title: Whole-exome sequencing unveils novel potential gene mutations involved in primary renal small cell carcinoma
    Article Snippet: .. The concentration was accurately measured using the Qubit ® dsDNA HS Assay Kit (Thermo Fisher Scientific) and confirmed by quantitative PCR (qPCR) using the KAPA Library Quantification Kit (Roche, Catalog No. KK4824) to ensure precise molarity calculation for cluster generation. ..

    Article Title: Cell culture methods and compositions
    Article Snippet: To remove excess primers and adaptor dimers, PCR products were purified twice using SPRI beads (Beckman Coulter). .. The quality of the libraries was assessed using Agilent Bioanalyzer DNA 1000 chip (Duke-NUS Genome Facility) and the molar concentration of each library was determined using KAPA library quantification kit (Roche). .. Three pooled libraries (each containing 9 samples with the pooled concentration of 8 nM in 30 uL) was sent for sequencing on Illumina HiSeq4000 (NovogeneAIT Genomics Singapore).



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